vitrification and subsequent in vitro maturation of mouse preantral follicles in presence of growth factors
نویسندگان
چکیده
objective: cryopreservation of ovarian tissue or follicles has been proposed as an alternative method for fertility preservation. although successful vitrification of follicles has been reported in several mammalian species, the survival rate is generally low. the aim of this study was to investigate the effects of fibroblast growth factor (fgf) and epidermal growth factor (egf) on in vitro preantral follicle development after vitrification. materials and methods:in this experimental study, preantral follicles with diameter of 150-180 μm were mechanically isolated from ovaries of 18-21 days old nmri mice. follicles were vitrified and warmed, then cultured in α-minimal essential medium (α-mem) without growth factor supplementation as control group (group i), while supplemented with 20 ng/ml fgf (group ii), 20 ng/ml egf (group iii), and 20 ng/ml fgf +20 ng/ml egf (group iv). after 12 days, human chorionic gonadotrophin (hcg)/egf was added to culture medium, and after 18-20 hours, the presence of cumulus oocyte complexes (cocs) and oocyte maturation were assessed. the chi-square (χ2) test was used to analyze survival and ovulation rates of the follicles. results: our results showed that the rate of metaphase ii (mii) oocytes in fgf group increased in comparison with control and other treatment groups (p<0.027), but there was no difference between control with egf and egf+fgf groups in oocyte maturation rate (p>0.05). there was a significant decrease in survival rate of follicles in egf+fge group in comparison with other groups (p<0.008). after in vitro ovulation induction, the follicles in egf group showed a higher ovulation rate (p<0.008) than those cultured in other groups. conclusion: fgf has beneficial effect on oocyte maturation, and egf increases cocs number in vitro. combination of egf and fge decreases the number of survived follicles.
منابع مشابه
Vitrification and Subsequent In Vitro Maturation of Mouse Preantral Follicles in Presence of Growth Factors
OBJECTIVE Cryopreservation of ovarian tissue or follicles has been proposed as an alternative method for fertility preservation. Although successful vitrification of follicles has been reported in several mammalian species, the survival rate is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and epidermal growth factor (EGF) on in vitro prea...
متن کاملP-107: Vitrification and In Vitro Culture of Mouse Preantral Follicles in Presence of Growth Factors
Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...
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Melatonin Impact on In Vitro Development of Mouse Preantral Follicles and Oocyte Maturation
Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diamet...
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عنوان ژورنال:
cell journalجلد ۱۶، شماره ۳، صفحات ۲۷۱-۲۷۸
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